POLS Labs

Welcome to the POLS lab website.  Resources will be added continually throughout the semester.  Until then, watch some videos, read some blog posts, and read the syllabus to get oriented with this semesters’ project.

• A “textbook” is available for download on the Moodle page for this course.
• The SEA-PHAGES program has a website containing tons of great information.
• Phage Intro Video

Resources

• POLS Lab Syllabus – BIOB 161 – 2018
• POLS Lab Book Grading Rubric
• POLS Lab Poster Advice

Life and Biology (LaB) Videos

• Lab Book Advice (parts 1 and 2)
• Purification: Streak plates vs. spot tests

HHMI SEA-PHAGES Videos

• Phage Discovery Guide: Direct Isolation (Protocols 5.1 and 5.2)
• Phage Discovery Guide: Plaque Assay (Protocol 5.3)
• Phage Discovery Guide: Picking a Plaque (Protocol 5.4) 
• Phage Discovery Guide: Enriched Isolation (Protocol 5.5)
• Phage Discovery Guide: Spot Tests (Protocol 5.6)
• Phage Discovery Guide: Serial Dilution (Protocol 6.2)
• Phage Discovery Guide: Collecting Plate Lysate (Protocol 6.3)
• Phage Discovery Guide: DNA Extraction (Protocol 9.1)
• Bacteriophage “Why” Videos for SEA-PHAGES Program (parts 1, 2, and 3)