Page 2 – Life and Biology

Week 1 – Brittnee and Kurtiss

Submitted by Brittnee Crane for TRIM Proteins Kurtiss observed as I finished wrapping up a Yeast two-hybrid experiment. I had already ran through the entire protocol, picked yeast colonies that…

Discover Biology Lab…

Why work with Escherichia coli (E. coli)?

25 May 201829 May 2018

For Discover Biology Lab (Background Information) It would probably be difficult to find a molecular biology laboratory that didn't have a freezer full of E. coli. Species that are commonly…

General research

Technique: Recombination Using NEBuilder HiFi DNA Assembly Master Mix

For Techniques Links Product site NEBuilder HiFi DNA Assembly Cloning Kit

General research…

Week 2 – Hannah, Camille, and Kristine

For TRIM Proteins Accomplishments: Discovery of TRIM8 and TRIM13v3 gel purified PCR products in the freezer. Check gels confirmed the presence of these TRIMS in the correct place. Strong band of…

LaB YouTube Video…

Ubiquitin Enzymatic Cascade: Role of E3 Ligase

Our laboratory studies E3 ubiquitin ligase enzymes. This video puts these E3 enzymes into the context of the ubiquitin enzyme cascade that consists of E1, E2, and E3 enzymes as…

Summer Team To Do (List 2)

For TRIM Proteins Research Review the first to do list and discuss your progress with your group Write up a progress report summarizing accomplishments and works in progress (see Hannah's first report,…

Questions to be answered using Y2H

When you clone a gene into pGBKT7 and have confirmed that it is the exact sequence you think it is, the next thing you'll want to do is transform it…

Week 1 – Hannah, Camille, and Kristine

Week 1 Lab Summary (5/7/18-5/14/18) This week my team and I setup a digest for pGADT7-TRIM5α, from a miniprep done by J. Graff previously in the semester. The digest from…