Protein analysis (part 3)

Biotechnology Chapter 9 Day 3 Questions

1. How does the yeast two-hybrid system work?
2. Examine figures 6 and 7 from this paper.  Draw each panel and explain the experimental design as well as the interpretation of the co-immunoprecipitation data.
3. Experiments are designed to answer questions that researchers have.  What type of questions would be answered using an antigen capture immunoassay?  A direct immunoassay array?
4. In experiments using protein interaction arrays, what types of molecules could “bind to” the spotted proteins?
5. Discuss the analogy that networks of metabolites can be compared to city streets.