- Agarose gel electrophoresis is a technique used in biology, chemistry and genetics to separate different types of molecules like proteins and DNA.
- It works by running an electric current through a matrix of agarose that is placed in a gel box containing a solution of salts that allow electrical current to flow through the solution.
- The molecules can either be separated by their charge, which is called isoelectric focusing, or, in the case of DNA and RNA, by their length.
- The separated DNA can be seen under UV light and can then be taken out of the gel. Agarose gels are usually 0.7-2% dissolved in a special electrophoresis buffer.
How is agarose gel electrophoresis used to separate molecules?