Exploring the Basics of Agarose Gel Electrophoresis

  • Agarose gel electrophoresis is a technique used in biology, chemistry and genetics to separate different types of molecules like proteins and DNA.
  • It works by running an electric current through a matrix of agarose that is placed in a gel box containing a solution of salts that allow electrical current to flow through the solution.
  • The molecules can either be separated by their charge, which is called isoelectric focusing, or, in the case of DNA and RNA, by their length.
  • The separated DNA can be seen under UV light and can then be taken out of the gel. Agarose gels are usually 0.7-2% dissolved in a special electrophoresis buffer.
        three DNA molecules running in a race | pencil sketch with lots of details

          How is agarose gel electrophoresis used to separate molecules?

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