For Standard operating procedures Congratulations!  But...before celebrating too much, make sure to check that your insert is what you think it is.  Here are things you should work on now: Pick a colony or two from the plate and spot some of the bacteria onto another plate (aka masterplate) and shake the rest of the bacteria … Continue reading My cloning attempt into a plasmid yielded colonies following transformation. Now what?

A one-minute summary of "Systematic discovery of antiphage defense systems in the microbial pangenome" by Doron et. al Some of the most useful tools in the molecular biologists toolkit come from using defense systems of prokaryotes (bacteria and archaea). These defenses protect the prokaryotes from bacteriophages (aka "phages" - viruses that infect bacteria). A new study in … Continue reading New Antiviral Defense Systems Discovered