Protocol 6.3: Collecting Phage Lysate

For POLS Lab Once you have taken your bacteriophage sample through two rounds of purification, it is time to amplify your clonal virus population.  This process involves first determining the titer of your purified phage.  (We will use a spot test of serially-diluted samples for this.)  Then, using the titer information, we will perform full-plate … Continue reading Protocol 6.3: Collecting Phage Lysate

Mystery Solved: How Lymphocyte Receptors are Encoded by a Few Genes

For Immunology Chapter 7 Day 1 Compare germline and somatic hypermutation theories. Hozumi and Tonegawa tested the idea that Ig light chains were a mosaic of variable and constant domains.  What does this mean? What was Hozumi and Tonegawa’s method for testing this? What cells did they use?  Why? How did their assay work? Can … Continue reading Mystery Solved: How Lymphocyte Receptors are Encoded by a Few Genes