Biotechnology Chapter 5 Day 2 Questions What are morpholinos? What would happen if you injected morpholinos into a frog egg? Discuss how siRNAs can “spread” systemically through a plant. What is PTGS? Describe miRNA maturation starting with transcription and ending with binding of an Argonaute protein to the target mRNA(s). A new category of RNA … Continue reading RNAi, PTGS, siRNA, miRNA, shRNA, but not piRNA…that was last time
Author: Life and Biology
Phage Discovery Guide: Spot Tests
A good time to test for the presence of bacteriophage in your sample using this method is after you have collected your enriched isolation samples. Rather than performing a plaque assay using serial dilutions, a spot test can be performed with just a single plate. The spot test can be used as a yes or no … Continue reading Phage Discovery Guide: Spot Tests
A Tale of Leishmania Immunity in Multiple NLRP10 Knockout Mice
Congratulations to my friend and co-worker for many years, Gwen Clay. This week an interesting study was published in the Journal of Immunology evaluating the role of a protein in the NOD-like receptor family (NLRP10) during cutaneous leishmaniasis. Gwen and I had countless discussions about data interpretation that were made all the more complicated upon the … Continue reading A Tale of Leishmania Immunity in Multiple NLRP10 Knockout Mice
Life and Biology 