Biotechnology Chapter 4 Day 3 Questions Most of the PCR protocols we’ve learned about using two oligonucleotides and overlap PCR required three oligonucleotides. Why does a sequencing PCR require one single primer? Would you expect a Sanger sequencing reaction to have equal numbers of deoxynucleotides and dideoxynucleotides? If not, which type of nucleotide should be … Continue reading Sanger Sequencing vs. Next-Generation Sequencing