Biotechnology Chapter 3 Day 3 Cake post questions Why would a researcher use a “poly(T)” column during expression library construction? What is the purpose of adding linkers to the double-stranded DNA? Compare and contrast the method of regulating the lacUV and lambda left promoters. Getting bacteria to take up linear, nonreplicable (no ori sequence) DNA … Continue reading Biotechnology – Gene Expression Libraries and Cloning Strategies

My birthday cake from last year should be recognizable to this year's biotech students.  In which bacterial colonies has the alpha fragment of the beta-galactosidase gene been interupted with a DNA insert?  What is the name of beta-galactosidase substrate added to the agar medium?

Biotechnology Chapter 3 Day 2 (pages 73-85) Genome browsers, such as the USCS Genome Browser, provide GC% content information. In other words, it shows information about whether guanines and cytosines are enriched in different stretches of genomic DNA.  One reason is related to genetic features called CpG islands.  Another reason for this information is related … Continue reading Biotechnology Chapter 3 Day 2 Questions