Biotechnology – Gene Expression Libraries and Cloning Strategies

Biotechnology Chapter 3 Day 3

1. Cake post questions
2. Why would a researcher use a “poly(T)” column during expression library construction?
3. What is the purpose of adding linkers to the double-stranded DNA?
4. Compare and contrast the method of regulating the lacUV and lambda left promoters.
5. Getting bacteria to take up linear, nonreplicable (no ori sequence) DNA is not futile in recombineering. Why?

Primer Design for NEBuilder HiFi Assembly

Cloning using Gibson assembly

Cloning using Golden Gate Assembly