Biotechnology DNA Synthesis (In Vivo and In Vitro)

Biotechnology Chapter 4 Day 1 Questions

  1. How does DNA methylase know where to add methyl groups to the new strand of DNA following replication?
  2. Draw figure 4.2 with as much detail as possible.  Describe the role of each enzyme including the subunits of DNA pol III.
  3. What is the connection between lagging strand synthesis during DNA replication and telomeres?
  4. What’s the purpose of the RNA made by primase?
  5. Why does replicating DNA in vivo (in a cell) require more “reagents” than in vivo (in a lab reaction)-based techniques?
  6. Write a step-by-step explanation of oligonucleotide (“oligo”) DNA synthesis starting with the first nucleotide attaching to CPG (controlled pore glass).
  7. Why is it critical for DNA synthesizers to have an efficiency of 99% or better when making a typical oligo?

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