Biotechnology Chapter 5 Day 1 Questions What is a ribozyme? What does the hammerhead ribozyme do and where did it get its name? How does our definition of ribozyme get violated by hammerhead’s activity? Name a ribozyme that doesn’t violate our ribozyme definition. Piwi-interacting RNAs are highly expressed in testes. Given the textbook’s description of … Continue reading The Big World of RNAs
Category: Biotechnology course
Sanger Sequencing vs. Next-Generation Sequencing
Biotechnology Chapter 4 Day 3 Questions Most of the PCR protocols we’ve learned about using two oligonucleotides and overlap PCR required three oligonucleotides. Why does a sequencing PCR require one single primer? Would you expect a Sanger sequencing reaction to have equal numbers of deoxynucleotides and dideoxynucleotides? If not, which type of nucleotide should be … Continue reading Sanger Sequencing vs. Next-Generation Sequencing
PCR (and PCR Variations)
Biotechnology Chapter 4 Day 2 Study Questions A thermocycler is an instrument that changes the temperature of your PCRs. What is the purpose for each of the three cycled temperatures? Why is the lowest temperature during the PCR cycling suggested to be in the 50-60C range "depending on the length and sequence of the primer?" … Continue reading PCR (and PCR Variations)
Life and Biology 