In this video, I introduce genome editing and focus on how the CRISPR-Cas system can be used to precisely cut a genome in the correct location. Relevant links: A single guide RNA can be formed with a linker connecting the crRNA and the tracrRNA: http://www.sciencemag.org/content/337... (Charpentier/Doudna labs) Proof of concept: Genome editing in human cells: … Continue reading CRISPR: Genome Editing Basics

This is the first episode about the CRISPR system in bacteria, but it won't be the last. Here, I discuss an important paper that proved the acquisition of bacteriophage sequence and integration of that sequence into a CRISPR locus provides bacteria with resistance to the bacteriophage. 2007 (not 2006) paper in Science by Barrangou et … Continue reading CRISPR: Acquired Bacterial Immune Systems

Initial genetic characterization of the 1918 “Spanish” influenza virus. 1997. Science. http://www.ncbi.nlm.nih.gov/pubmed/90... Characterization of the 1918 influenza virus polymerase genes. 2005. Nature. http://www.ncbi.nlm.nih.gov/pubmed/16... Characterization of the reconstructed 1918 Spanish influenza pandemic virus. 2005. Science http://www.ncbi.nlm.nih.gov/pubmed/16... J Kaiser commentary Resurrected influenza virus yields secrets of deadly 1918 pandemic. http://www.ncbi.nlm.nih.gov/pubmed/16... https://youtu.be/qdm-RiQ33lk