CRISPR: Genome Editing Basics

In this video, I introduce genome editing and focus on how the CRISPR-Cas system can be used to precisely cut a genome in the correct location.

Relevant links:

• A single guide RNA can be formed with a linker connecting the crRNA and the tracrRNA: http://www.sciencemag.org/content/337… (Charpentier/Doudna labs)

Proof of concept:

• Genome editing in human cells:
  • http://cdn.elifesciences.org/elife-ar… (Doudna lab)
  •  http://www.sciencemag.org/content/339… (Church lab) http://www.sciencemag.org/content/339… (Zhang lab)