Our laboratory studies E3 ubiquitin ligase enzymes. This video puts these E3 enzymes into the context of the ubiquitin enzyme cascade that consists of E1, E2, and E3 enzymes as well as ubiquitin and target proteins. The goal for our lab is to identify proteins that the E3 enzymes interact with to inform future studies … Continue reading Ubiquitin Enzymatic Cascade: Role of E3 Ligase
Category: TRIM Proteins
Questions to be answered using Y2H
When you clone a gene into pGBKT7 and have confirmed that it is the exact sequence you think it is, the next thing you'll want to do is transform it into yeast. We have a kit for that and the manual is here: PT1172-1. Some immediate needs are as follows: Make a glycerol stock. Just like … Continue reading Questions to be answered using Y2H
Tool: Finding Splice Variants
Previously, we have used a laborious method to identify splice variants of the various TRIM proteins. Moreover, we have not had a good method to quickly figure out what protein isoforms are possible from the splice variants. Our lives just got easier with the discovery of SpliceMiner. Just go to this website and type in … Continue reading Tool: Finding Splice Variants
Life and Biology 