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- 100 mL 10X Tris-glycine
- 10 mL 10% SDS (w/v in water)
Bring total volume up to 1 liter.
Note: To save on buffer, I usually store used buffer in a well-labeled bottle. Each new SDS-PAGE protein gel is set up with fresh 1X SDS-PAGE running buffer in the internal reservoir of the electrophoresis apparatus and used 1X SDS-PAGE running buffer in the outer chamber of the apparatus.