6X Loading Buffer (protein)

Materials and recipes

  • 15 mL 1M Tris-Cl (pH 6.8)
  • 6 g SDS
  • Bromophenol blue (to taste)
  • 30 mL Glycerol
  • 2 mL Betamercaptomethanol (14.3 M stock concentration)

This recipe should add up to approximately 50 mL total volume.  If not, add water to 50 mL.  Store 10 mL aliquots of this 6X stock in the freezer and make 0.5 mL aliquots from the 10 mL aliquots as the buffer is used up.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s