Cell Analyses Using Antibodies

Biotechnology Chapter 6 Day 2

  1. Antibody variable regions are of particular interest in terms of biotechnology applications. Discuss how Fab fragments, dsFv fragments, and scFv fragments attempt to shed excess protein sequence.
  2. What’s so great about camel antibodies?
  3. What could bispecific antibodies be used for?
  4. Look at this paper and discuss how this new technique is a spinoff of techniques we’ve been discussing including aptamers, antibody fragments, and phage display.
  5. Some ELISA assays use one antibody (called a primary antibody) and others use two antibodies (a primary antibody and a secondary antibody). Why is the second method more versatile and less expensive in the long run?
  6. How does a home pregnancy test work?
  7. What’s the difference between immunocytochemistry and immunohistochemistry?
  8. How does flow cytometry work and how do you read the data for one color-, two color-, and three color-samples?
  9. What are two “free” data points you discover about each cell during flow cytometry that are based solely on light scatter?
  10. What are some differences between FACS and MACS?
  11. What is CyTOF?
  12. What is multidimensional data visualization?

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