Biotechnology Chapter 8 Day 2 Questions
- Briefly discuss whole-genome tiling arrays, quasi-whole-genome arrays, splice junction arrays, and exon scanning arrays.
- For humans, only a chromosome 21 and chromosome 22 whole genome array is available. Why these chromosomes? What did the book mention was learned from this platform? What technology has supplanted whole genome arrays?
- Compare ChIP-chip and ChIP-seq.
- What is the outcome of having a gene that is heavily methylated? View the bisulfate-treated DNA results in figure 1 of the Nobel-prize winning work for the creating induced pluripotent stem cells (iPSCs). What would you conclude about the variety of genes that are active in embryonic stem cells, iPSCs, and fibroblasts?
- Briefly discuss each of the major advantages of RNA-seq (a-h). What were the disadvantages of RNA-seq compared to whole-genome tiling arrays? Can these disadvantages be mitigated?
- Most of the time, scientists use “Poly(T) beads” in their workflow for RNA-seq. Why? What could be missed by including this step?
- How does a reporter gene work? How can this concept be modified to report the activity of a specific transcription factor?