For POLS Labs The number of infectious bacteriophages in your stocks may seem absurdly high. For example, for some experiments, we may expect there to be 1-100 billion (1e9 to 1e11) phage per milliliter of your stock. To be able to "count" the number of infectious bacteriophages are in your sample, you will need to dilute … Continue reading Phage Discovery Guide: Serial Dilution Protocol
Author: Life and Biology
Intro to Immunology Questions
For immunology Chapter 1 Day 1 In what ways are immunity and vaccination closely related? What characteristic of rabies infection allows for the vaccination to be efficacious even though the person has already been infected? How was the rabies vaccine prepared? (The answers to these questions are not fully answered in the book.) No vaccine … Continue reading Intro to Immunology Questions
Phage Discovery Guide: Picking a Plaque Protocol
For POLS Labs This video shows you how easy it is to "pick a plaque". You will perform this technique several times during the semester. It will be particularly important during the phage isolation and purification portions of the curriculum. Perform this assay only with well-isolated plaques from a plaque assay. Do not perform this assay … Continue reading Phage Discovery Guide: Picking a Plaque Protocol
Life and Biology 