For Biotechnology course Describe a couple of reasons why cDNA is cloned into plasmids when using bacterial protein expression systems rather than genomic sequence for a particular gene. How could you remedy a situation where the 5' end of a cloned gene forms a hairpin structure with the Shine-Delgarno sequence? Insulin is first created as … Continue reading Recombinant Protein Technologies (Part 1)

Biotechnology Chapter 9 Day 3 Questions How does the yeast two-hybrid system work? Examine figures 6 and 7 from this paper.  Draw each panel and explain the experimental design as well as the interpretation of the co-immunoprecipitation data. Experiments are designed to answer questions that researchers have.  What type of questions would be answered using an … Continue reading Protein analysis (part 3)

Biotechnology Chapter 9 Day 2 Questions If you were to kick a soccer ball as hard as you could, how many seconds would it likely take for it to travel 10 feet? If you were to kick a bowling ball as hard as you could, how many seconds would it likely take for it to … Continue reading Protein analysis (part 2)