Biotechnology Chapter 9 Day 1 Questions Note: As usual, some of the questions go beyond the information that the book has provided.  Google is your friend. When performing a two-dimensional PAGE experiment, why would you use a zwitterionic detergent during the first-dimensional separation and a negatively-charged detergent, such as SDS, for the second dimension? Some … Continue reading Protein analysis (part 1)

Biotechnology Chapter 8 Day 2 Questions Briefly discuss whole-genome tiling arrays, quasi-whole-genome arrays, splice junction arrays, and exon scanning arrays. For humans, only a chromosome 21 and chromosome 22 whole genome array is available. Why these chromosomes?  What did the book mention was learned from this platform?  What technology has supplanted whole genome arrays? Compare … Continue reading Chips, ChIP-chips, ChIP-seqs, other seqs, and, um, reporter genes

Biotechnology Chapter 6 Day 2 Antibody variable regions are of particular interest in terms of biotechnology applications. Discuss how Fab fragments, dsFv fragments, and scFv fragments attempt to shed excess protein sequence. What’s so great about camel antibodies? What could bispecific antibodies be used for? Look at this paper and discuss how this new technique … Continue reading Cell Analyses Using Antibodies