Biotechnology Chapter 4 Day 1 Questions How does DNA methylase know where to add methyl groups to the new strand of DNA following replication? Draw figure 4.2 with as much detail as possible.  Describe the role of each enzyme including the subunits of DNA pol III. What is the connection between lagging strand synthesis during … Continue reading Biotechnology DNA Synthesis (In Vivo and In Vitro)

Biotechnology Chapter 3 Day 3 Cake post questions Why would a researcher use a “poly(T)” column during expression library construction? What is the purpose of adding linkers to the double-stranded DNA? Compare and contrast the method of regulating the lacUV and lambda left promoters. Getting bacteria to take up linear, nonreplicable (no ori sequence) DNA … Continue reading Biotechnology – Gene Expression Libraries and Cloning Strategies

My birthday cake from last year should be recognizable to this year's biotech students.  In which bacterial colonies has the alpha fragment of the beta-galactosidase gene been interupted with a DNA insert?  What is the name of beta-galactosidase substrate added to the agar medium?