Materials and recipes 15 mL 1M Tris-Cl (pH 6.8) 6 g SDS Bromophenol blue (to taste) 30 mL Glycerol 2 mL Betamercaptomethanol (14.3 M stock concentration) This recipe should add up to approximately 50 mL total volume.  If not, add water to 50 mL.  Store 10 mL aliquots of this 6X stock in the freezer … Continue reading 6X Loading Buffer (protein)

Back to Resource Library 100 mL 10X Tris-glycine 10 mL 10% SDS (w/v in water) Bring total volume up to 1 liter. Note: To save on buffer, I usually store used buffer in a well-labeled bottle.  Each new SDS-PAGE protein gel is set up with fresh 1X SDS-PAGE running buffer in the internal reservoir of … Continue reading 1X SDS-PAGE Running Buffer

Materials and recipes This is a component of 1X SDS-PAGE running buffer. 30 g Tris-Cl 144 g Glycine Dissolve in 800 mL of water and then bring volume up to 1 liter.