Course: Introduction to Evolution As the summer winds down, it is time to start thinking about this fall's classes. My personal favorite class to teach is "Introduction to Evolution". We will spend 15 weeks digging through Carl Zimmer's "The Tangled Bank" textbook. Topics to be discussed include: Geology Phyologeny Genetics Mutation Various selective pressures Speciation … Continue reading Evolution Introductory Videos
Author: Life and Biology
6X Loading Buffer (protein)
Materials and recipes 15 mL 1M Tris-Cl (pH 6.8) 6 g SDS Bromophenol blue (to taste) 30 mL Glycerol 2 mL Betamercaptomethanol (14.3 M stock concentration) This recipe should add up to approximately 50 mL total volume. If not, add water to 50 mL. Store 10 mL aliquots of this 6X stock in the freezer … Continue reading 6X Loading Buffer (protein)
1X SDS-PAGE Running Buffer
Back to Resource Library 100 mL 10X Tris-glycine 10 mL 10% SDS (w/v in water) Bring total volume up to 1 liter. Note: To save on buffer, I usually store used buffer in a well-labeled bottle. Each new SDS-PAGE protein gel is set up with fresh 1X SDS-PAGE running buffer in the internal reservoir of … Continue reading 1X SDS-PAGE Running Buffer
Life and Biology 